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To identify differential metabolic features between the groups, we first adjusted for confounding factors (e.g., diabetes, hyperlipidemia, anxiety and depression, and the use of anti-Parkinsonian drugs) via logistic regression

Then, careful layering of 2.6 mL each of 1.15 M, 0.86 M, and 0.25 M sucrose solutions was performed on the microsomal suspension, and this step gradient sucrose solution was centrifugated at 82,000 g for 3 h at 4 C

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The structurally-related compound LVX was classified as a generally well tolerated non-DILI drug 20 associated with a substantially lower reporting rate of liver adverse effects compared to TVX 21

Essentially, 100 l of sample or Sphingomyelinase standards were added to the 96-well plate, together with an equal volume of the Reaction mixture, incubated at 37 C for 4h and then read on a FLUOStar Omega plate reader at 595 nm

A study published in 2003 [13] demonstrated that glutathione has anti-influenza properties