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DCFH-DA is internalized by the cell and then deacetylated by cellular esterases to generate 2',7'-dichlorodihydrofluorescein (DCFH), which is then oxidized by ROS to yield the green fluorescent compound 2',7'-dichlorofluorescein (DCF) [39]

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Journal of Investigative Dermatology, 125(5), 101-105

This cascade controls nitric oxide production and blood vessel formation