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Western blotting was performed to determine the effectiveness of the siRNAs

Coincidental timing is perhaps the most common explanation

In vivo protective effects of ferulic acid ethyl ester against amyloid-beta peptide 142-induced oxidative stress

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Pellets were resuspended in 100 L sample buffer with the addition of 5% -mercaptoethanol and analyzed by gel electrophoresis and immunoblotting, as described above
