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In addition, the immunopositive stained area percentages for MYD88, TLR4, and NF-B were evaluated using the same software applying the color deconvolution plugin and H-DAB vector ( 2.10 Immunoblotting A tissue homogenate was prepared from the testicular tissue, and the protein content was ascertained using the Bradford Protein Assay kit (Bio Basics Inc., Ontario, Canada, Cat# SK3041)

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Patients with a history of chronic hepatitis B or hepatitis C virus infection, HIV/AIDS, TB, uncontrolled T2DM, SARS-CoV2 infection within 6 months, and a history of alcohol abuse within 6 months were excluded

These were then selected further using the one-way analysis of variance (ANOVA) test, followed by a correction for multiple comparisons by controlling the false discovery rate with the two-stage step-up method of Benjamini, Krieger, and Yekutieli, as recommended by the GraphPad Prism software (version 7.0 for Mac OS X)